Title

Biomarkers in Tissue Samples From Young Patients With Acute Myeloid Leukemia
Promoter Methylation in MLL-Rearranged Childhood AML
  • Phase

    N/A
  • Study Type

    Observational
  • Status

    Completed No Results Posted
  • Intervention/Treatment

    DNA methylation analysis gene expression analysis microarray analysis reverse transcriptase-polymerase chain reaction laboratory biomarker analysis
  • Study Participants

    32
RATIONALE: Studying samples of tissue from patients with cancer the in laboratory may help doctors identify and learn more about biomarkers related to cancer.

PURPOSE: This research study is studying biomarkers in tissue samples from young patients with acute myeloid leukemia.
OBJECTIVES:

To determine whether the pattern of global and TSG-specific promoter CpG island hypermethylation and gene silencing that we have shown characterizes MLL-rearranged (MLL-r) infant bilineage ALL is also characteristic of other subsets of MLL-r leukemia.

OUTLINE: Previously collected cryopreserved cells from diagnosis are analyzed for promoter methylation via HELP arrays, gene expression arrays, and RT-qPCR.

PROJECTED ACCRUAL: A total of 32 samples (8 from each of 4 biologically defined cohorts) will be analyzed.
Study Started
Nov 30
2010
Primary Completion
May 31
2016
Last Update
May 19
2016
Estimate

Genetic DNA methylation analysis

Genetic gene expression analysis

Genetic microarray analysis

Genetic reverse transcriptase-polymerase chain reaction

Other laboratory biomarker analysis

Population

Patients With Acute Myeloid Leukemia.

Criteria

DISEASE CHARACTERISTICS:

Available cryopreserved cells from diagnosis

At least 2 x 10^7 viably cryopreserved cells

One of the following biologically defined cytogenetics/molecular cohorts:

t(9;11)
t(11;19)
Other 11q23 translocations
Normal cytogenetics

PATIENT CHARACTERISTICS:

Not specified

PRIOR CONCURRENT THERAPY:

Not specified
No Results Posted